Electrode-Respiring Microbiomes Associated with the Enhanced Bioelectrodegradation Function

  • Bin Liang
  • Mengyuan Qi
  • Hui Yun
  • Youkang Zhao
  • Yang Bai
  • Deyong Kong
  • Ai-Jie Wang


Microbial electrode-respiration process has been proved to significantly enhance the microbial oxidation or microbial reduction of various hazardous organic contaminants in bioelectrochemical systems (BESs). The microbial ecology and physiology of the involved electrode-associated multispecies biofilms are essential for the catalytic function of BESs. In this chapter, we summarize the advances of the electrode-respiring biofilm microbiomes involved in the catalysis of various hazardous organic contaminants at both the cathode and the anode sides. We also highlight the challenges and outlook for the electrode-respiring biofim microbiomes research from the microbial ecology perspective. Understanding the comprehensive information of the electrode-respiring microbiomes, including biofilm structure, composition, dynamics, activity, diversity, potential functional microbes, and interaction, is potentially feasible for regulating and scaling-up the microbial electrode-respiration-based engineering systems as well as the management of bioremediation applications.


Electrode-respiring microbiome Bioanode community Biocathode community Bioelectrorespiration Bioelectrodegradation Microbial ecology 



This work was supported by the National Natural Science Foundation of China (No. 31500084) and the Key Research Program of the Chinese Academy of Sciences (No. KFZD-SW-219).


  1. 1.
    Wang H, Ren ZJ (2013) A comprehensive review of microbial electrochemical systems as a platform technology. Biotechnol Adv 31(8):1796–1807. Scholar
  2. 2.
    Wang H, Luo H, Fallgren PH, Jin S, Ren ZJ (2015) Bioelectrochemical system platform for sustainable environmental remediation and energy generation. Biotechnol Adv 33(3):317–334. Scholar
  3. 3.
    Yun H, Liang B, Kong DY, Cheng HY, Li ZL, Gu YB, Yin HQ, Wang AJ (2017) Polarity inversion of bioanode for biocathodic reduction of aromatic pollutants. J Hazard Mater 331:280–288. Scholar
  4. 4.
    Daghio M, Aulenta F, Vaiopoulou E, Franzetti A, Arends JB, Sherry A, Suarez-Suarez A, Head IM, Bestetti G, Rabaey K (2017) Electrobioremediation of oil spills. Water Res 114:351–370. Scholar
  5. 5.
    Cheng HY, Liang B, Mu Y, Cui MH, Li K, Wu WM, Wang AJ (2015) Stimulation of oxygen to bioanode for energy recovery from recalcitrant organic matter aniline in microbial fuel cells (MFCs). Water Res 81:72–83. Scholar
  6. 6.
    Cui MH, Cui D, Gao L, Wang AJ, Cheng HY (2016) Azo dye decolorization in an up-flow bioelectrochemical reactor with domestic wastewater as a cost-effective yet highly efficient electron donor source. Water Res 105:520–526. Scholar
  7. 7.
    Kong D, Yun H, Cui D, Qi M, Shao C, Cui D, Ren N, Liang B, Wang A (2017) Response of antimicrobial nitrofurazone-degrading biocathode communities to different cathode potentials. Bioresour Technol 241:951–958. Scholar
  8. 8.
    Liang B, Cheng H, Van Nostrand JD, Ma J, Yu H, Kong D, Liu W, Ren N, Wu L, Wang A, Lee DJ, Zhou J (2014) Microbial community structure and function of nitrobenzene reduction biocathode in response to carbon source switchover. Water Res 54:137–148. Scholar
  9. 9.
    Liang B, Cheng HY, Kong DY, Gao SH, Sun F, Cui D, Kong FY, Zhou AJ, Liu WZ, Ren NQ, Wu WM, Wang AJ, Lee DJ (2013) Accelerated reduction of chlorinated nitroaromatic antibiotic chloramphenicol by biocathode. Environ Sci Technol 47(10):5353–5361. Scholar
  10. 10.
    Liang B, Kong D, Ma J, Wen C, Yuan T, Lee DJ, Zhou J, Wang A (2016) Low temperature acclimation with electrical stimulation enhance the biocathode functioning stability for antibiotics detoxification. Water Res 100:157–168. Scholar
  11. 11.
    Wang AJ, Cheng HY, Liang B, Ren NQ, Cui D, Lin N, Kim BH, Rabaey K (2011) Efficient reduction of nitrobenzene to aniline with a biocatalyzed cathode. Environ Sci Technol 45(23):10186–10193. Scholar
  12. 12.
    Wang L, Liu Y, Ma J, Zhao F (2016) Rapid degradation of sulphamethoxazole and the further transformation of 3-amino-5-methylisoxazole in a microbial fuel cell. Water Res 88:322–328. Scholar
  13. 13.
    Zhang Q, Zhang Y, Li D (2017) Cometabolic degradation of chloramphenicol via a meta-cleavage pathway in a microbial fuel cell and its microbial community. Bioresour Technol 229:104–110. Scholar
  14. 14.
    Guo K, Prevoteau A, Patil SA, Rabaey K (2015) Engineering electrodes for microbial electrocatalysis. Curr Opin Biotechnol 33:149–156. Scholar
  15. 15.
    Wang HC, Cheng HY, Cui D, Zhang B, Wang SS, Han JL, Su SG, Chen R, Wang AJ (2017) Corrugated stainless-steel mesh as a simple engineerable electrode module in bio-electrochemical system: hydrodynamics and the effects on decolorization performance. J Hazard Mater 338:287–295. Scholar
  16. 16.
    Cui D, Cui M-H, Lee H-S, Liang B, Wang H-C, Cai W-W, Cheng H-Y, Zhuang X-L, Wang A-J (2017) Comprehensive study on hybrid anaerobic reactor built-in with sleeve type bioelectrocatalyzed modules. Chem Eng J 330:1306–1315. Scholar
  17. 17.
    Cui D, Guo YQ, Cheng HY, Liang B, Kong FY, Lee HS, Wang AJ (2012) Azo dye removal in a membrane-free up-flow biocatalyzed electrolysis reactor coupled with an aerobic bio-contact oxidation reactor. J Hazard Mater 239–240:257–264. Scholar
  18. 18.
    Kong F, Wang A, Liang B, Liu W, Cheng H (2013) Improved azo dye decolorization in a modified sleeve-type bioelectrochemical system. Bioresour Technol 143:669–673. Scholar
  19. 19.
    Sun Q, Li Z, Wang Y, Cui D, Liang B, Thangavel S, Chung JS, Wang A (2015) A horizontal plug-flow baffled bioelectrocatalyzed reactor for the reductive decolorization of Alizarin Yellow R. Bioresour Technol 195:73–77. Scholar
  20. 20.
    Wang AJ, Cui D, Cheng HY, Guo YQ, Kong FY, Ren NQ, Wu WM (2012) A membrane-free, continuously feeding, single chamber up-flow biocatalyzed electrolysis reactor for nitrobenzene reduction. J Hazard Mater 199–200:401–409. Scholar
  21. 21.
    Cui D, Guo YQ, Lee HS, Wu WM, Liang B, Wang AJ, Cheng HY (2014) Enhanced decolorization of azo dye in a small pilot-scale anaerobic baffled reactor coupled with biocatalyzed electrolysis system (ABR-BES): a design suitable for scaling-up. Bioresour Technol 163:254–261. Scholar
  22. 22.
    Cui MH, Cui D, Gao L, Cheng HY, Wang AJ (2016) Efficient azo dye decolorization in a continuous stirred tank reactor (CSTR) with built-in bioelectrochemical system. Bioresour Technol 218:1307–1311. Scholar
  23. 23.
    Cui MH, Cui D, Lee HS, Liang B, Wang AJ, Cheng HY (2016) Effect of electrode position on azo dye removal in an up-flow hybrid anaerobic digestion reactor with built-in bioelectrochemical system. Sci Rep 6:25223. Scholar
  24. 24.
    Cui MH, Cui D, Gao L, Wang AJ, Cheng HY (2017) Evaluation of anaerobic sludge volume for improving azo dye decolorization in a hybrid anaerobic reactor with built-in bioelectrochemical system. Chemosphere 169:18–22. Scholar
  25. 25.
    Jiang X, Shen J, Han Y, Lou S, Han W, Sun X, Li J, Mu Y, Wang L (2016) Efficient nitro reduction and dechlorination of 2,4-dinitrochlorobenzene through the integration of bioelectrochemical system into upflow anaerobic sludge blanket: a comprehensive study. Water Res 88:257–265. Scholar
  26. 26.
    Chen H, Gao X, Wang C, Shao J, Xu X, Zhu L (2017) Efficient 2,4-dichloronitrobenzene removal in the coupled BES-UASB reactor: effect of external voltage mode. Bioresour Technol 241:879–886. Scholar
  27. 27.
    Chen L, Shao J, Chen H, Wang C, Gao X, Xu X, Zhu L (2018) Cathode potential regulation in a coupled bioelectrode-anaerobic sludge system for effective dechlorination of 2,4-dichloronitrobenzene. Bioresour Technol 254:180–186. Scholar
  28. 28.
    Koch C, Korth B, Harnisch F (2018) Microbial ecology-based engineering of microbial electrochemical technologies. Microb Biotechnol 11(1):22–38. Scholar
  29. 29.
    Hassan H, Jin B, Dai S, Ma T, Saint C (2016) Chemical impact of catholytes on Bacillus subtilis-catalysed microbial fuel cell performance for degrading 2,4-dichlorophenol. Chem Eng J 301:103–114. Scholar
  30. 30.
    Wang S, Huang L, Gan L, Quan X, Li N, Chen G, Lu L, Xing D, Yang F (2012) Combined effects of enrichment procedure and non-fermentable or fermentable co-substrate on performance and bacterial community for pentachlorophenol degradation in microbial fuel cells. Bioresour Technol 120:120–126. Scholar
  31. 31.
    Hassan H, Schulte-Illingheim L, Jin B, Dai S (2016) Degradation of 2,4-dichlorophenol by Bacillus subtilis with concurrent electricity generation in microbial fuel cell. Proc Eng 148:370–377. Scholar
  32. 32.
    Huang L, Sun Y, Liu Y, Wang N (2013) Mineralization of 4-chlorophenol and analysis of bacterial community in microbial fuel cells. Procedia Environ Sci 18:534–539. Scholar
  33. 33.
    Zhang D, Li Z, Zhang C, Zhou X, Xiao Z, Awata T, Katayama A (2017) Phenol-degrading anode biofilm with high coulombic efficiency in graphite electrodes microbial fuel cell. J Biosci Bioeng 123(3):364–369. Scholar
  34. 34.
    Zhang T, Tremblay P-L, Chaurasia AK, Smith JA, Bain TS, Lovley DR (2013) Anaerobic benzene oxidation via phenol in Geobacter metallireducens. Appl Environ Microbiol 79(24):7800–7806. Scholar
  35. 35.
    Wei G, Xia D, Li-Li W, Hong Y (2018) Isolation, selection, and biological characterization research of highly effective electricigens from MFCs for phenol degradation. Folia Microbiol 63(1):73–83. Scholar
  36. 36.
    Chen Z, Niu Y, Zhao S, Khan A, Ling Z, Chen Y, Liu P, Li X (2016) A novel biosensor for p-nitrophenol based on an aerobic anode microbial fuel cell. Biosens Bioelectron 85:860–868. Scholar
  37. 37.
    Friman H, Schechter A, Ioffe Y, Nitzan Y, Cahan R (2013) Current production in a microbial fuel cell using a pure culture of Cupriavidus basilensis growing in acetate or phenol as a carbon source. Microb Biotechnol 6(4):425–434. Scholar
  38. 38.
    Hassan H, Jin B, Donner E, Vasileiadis S, Saint C, Dai S (2018) Microbial community and bioelectrochemical activities in MFC for degrading phenol and producing electricity: microbial consortia could make differences. Chem Eng J 332:647–657. Scholar
  39. 39.
    Zhao H, Kong C-H (2018) Enhanced removal of p-nitrophenol in a microbial fuel cell after long-term operation and the catabolic versatility of its microbial community. Chem Eng J.
  40. 40.
    Zhang S, Song H-L, Yang X-L, Yang K-Y, Wang X-Y (2016) Effect of electrical stimulation on the fate of sulfamethoxazole and tetracycline with their corresponding resistance genes in three-dimensional biofilm-electrode reactors. Chemosphere 164:113–119. Scholar
  41. 41.
    Zhang Q, Zhang L, Wang H, Jiang Q, Zhu X (2017) Simultaneous efficient removal of oxyfluorfen with electricity generation in a microbial fuel cell and its microbial community analysis. Bioresour Technol 250:658–665. Scholar
  42. 42.
    Zhang E, Yu Q, Zhai W, Wang F, Scott K (2017) High tolerance of and removal of cefazolin sodium in single-chamber microbial fuel cells operation. Bioresour Technol 249:76–81. Scholar
  43. 43.
    Wang L, Wu Y, Zheng Y, Liu L, Zhao F (2015) Efficient degradation of sulfamethoxazole and the response of microbial communities in microbial fuel cells. RSC Adv 5(69):56430–56437. Scholar
  44. 44.
    Jiang X, Shen J, Xu K, Chen D, Mu Y, Sun X, Han W, Li J, Wang L (2018) Substantial enhancement of anaerobic pyridine bio-mineralization by electrical stimulation. Water Res 130:291–299. Scholar
  45. 45.
    Jiang X, Shen J, Lou S, Mu Y, Wang N, Han W, Sun X, Li J, Wang L (2016) Comprehensive comparison of bacterial communities in a membrane-free bioelectrochemical system for removing different mononitrophenols from wastewater. Bioresour Technol 216:645–652. Scholar
  46. 46.
    Rozendal RA, Jeremiasse AW, Hamelers HV, Buisman CJ (2008) Hydrogen production with a microbial biocathode. Environ Sci Technol 42(2):629–634CrossRefGoogle Scholar
  47. 47.
    Cheng KY, Ho G, Cord-Ruwisch R (2010) Anodophilic biofilm catalyzes cathodic oxygen reduction. Environ Sci Technol 44(1):518–525. Scholar
  48. 48.
    Cheng KY, Ho G, Cord-Ruwisch R (2011) Novel methanogenic rotatable bioelectrochemical system operated with polarity inversion. Environ Sci Technol 45(2):796–802. Scholar
  49. 49.
    Pous N, Carmona-Martínez AA, Vilajeliu-Pons A, Fiset E, Bañeras L, Trably E, Balaguer MD, Colprim J, Bernet N, Puig S (2016) Bidirectional microbial electron transfer: switching an acetate oxidizing biofilm to nitrate reducing conditions. Biosens Bioelectron 75:352–358. Scholar
  50. 50.
    Lovley DR (2011) Powering microbes with electricity: direct electron transfer from electrodes to microbes. Environ Microbiol Rep 3(1):27–35. Scholar
  51. 51.
    Yun H, Kong D, Liang B, Cui M, Li Z, Wang A (2016) Response of anodic bacterial community to the polarity inversion for chloramphenicol reduction. Bioresour Technol 221:666–670. Scholar
  52. 52.
    Yun H, Liang B, Kong D, Wang A (2018) Improving biocathode community multifunctionality by polarity inversion for simultaneous bioelectroreduction processes in domestic wastewater. Chemosphere 194:553–561. Scholar
  53. 53.
    Kong D, Liang B, Yun H, Cheng H, Ma J, Cui M, Wang A, Ren N (2015) Cathodic degradation of antibiotics: characterization and pathway analysis. Water Res 72:281–292. Scholar
  54. 54.
    Sun F, Liu H, Liang B, Song R, Yan Q, Wang A (2013) Reductive degradation of chloramphenicol using bioelectrochemical system (BES): a comparative study of abiotic cathode and biocathode. Bioresour Technol 143:699–702. Scholar
  55. 55.
    Guo N, Wang Y, Yan L, Wang X, Wang M, Xu H, Wang S (2017) Effect of bio-electrochemical system on the fate and proliferation of chloramphenicol resistance genes during the treatment of chloramphenicol wastewater. Water Res 117:95–101. Scholar
  56. 56.
    Guo N, Wang Y, Tong T, Wang S (2018) The fate of antibiotic resistance genes and their potential hosts during bio-electrochemical treatment of high-salinity pharmaceutical wastewater. Water Res 133:79–86. Scholar
  57. 57.
    Zhang J, Zhang Y, Quan X (2015) Bio-electrochemical enhancement of anaerobic reduction of nitrobenzene and its effects on microbial community. Biochem Eng J 94:85–91. Scholar
  58. 58.
    Roldan MD, Perez-Reinado E, Castillo F, Moreno-Vivian C (2008) Reduction of polynitroaromatic compounds: the bacterial nitroreductases. FEMS Microbiol Rev 32(3):474–500. Scholar
  59. 59.
    Marvin-Sikkema FD, de Bont JA (1994) Degradation of nitroaromatic compounds by microorganisms. Appl Microbiol Biotechnol 42(4):499–507CrossRefGoogle Scholar
  60. 60.
    Qi M, Liang B, Chen R, Sun X, Li Z, Ma X, Zhao Y, Kong D, Wang J, Wang A (2018) Effects of surface charge, hydrophilicity and hydrophobicity on functional biocathode catalytic efficiency and community structure. Chemosphere 202:105–110.
  61. 61.
    Zhang L, Jiang X, Shen J, Xu K, Li J, Sun X, Han W, Wang L (2016) Enhanced bioelectrochemical reduction of p-nitrophenols in the cathode of self-driven microbial fuel cells. RSC Adv 6(35):29072–29079. Scholar
  62. 62.
    Wang X, Xing D, Ren N (2016) p-nitrophenol degradation and microbial community structure in a biocathode bioelectrochemical system. RSC Adv 6(92):89821–89826. Scholar
  63. 63.
    Wang YZ, Wang AJ, Liu WZ, Kong DY, Tan WB, Liu C (2013) Accelerated azo dye removal by biocathode formation in single-chamber biocatalyzed electrolysis systems. Bioresour Technol 146:740–743. Scholar
  64. 64.
    Peng X, Pan X, Wang X, Li D, Huang P, Qiu G, Shan K, Chu X (2017) Accelerated removal of high concentration p-chloronitrobenzene using bioelectrocatalysis process and its microbial communities analysis. Bioresour Technol 249:844–850. Scholar
  65. 65.
    Feng H, Zhang X, Guo K, Vaiopoulou E, Shen D, Long Y, Yin J, Wang M (2015) Electrical stimulation improves microbial salinity resistance and organofluorine removal in bioelectrochemical systems. Appl Environ Microbiol 81(11):3737–3744. Scholar
  66. 66.
    Xu X, Shao J, Li M, Gao K, Jin J, Zhu L (2016) Reductive transformation of p-chloronitrobenzene in the upflow anaerobic sludge blanket reactor coupled with microbial electrolysis cell: performance and microbial community. Bioresour Technol 218:1037–1045. Scholar
  67. 67.
    Feng H, Wang Y, Zhang X, Shen D, Li N, Chen W, Huang B, Liang Y, Zhou Y (2017) Degradation of p-fluoronitrobenzene in biological and bioelectrochemical systems: differences in kinetics, pathways, and microbial community evolutions. Chem Eng J 314:232–239. Scholar
  68. 68.
    Liu D, Lei L, Yang B, Yu Q, Li Z (2013) Direct electron transfer from electrode to electrochemically active bacteria in a bioelectrochemical dechlorination system. Bioresour Technol 148:9–14. Scholar
  69. 69.
    Kumar R, Singh L, Wahid ZA, Din MFM (2015) Exoelectrogens in microbial fuel cells toward bioelectricity generation: a review. Int J Energy Res 39(8):1048–1067. Scholar
  70. 70.
    Galai S, Pérez de los Ríos A, Hernández-Fernández FJ, Kacem SH, Ramírez FM, Quesada-Medina J (2015) Microbial fuel cell application for azoic dye decolorization with simultaneous bioenergy production using Stenotrophomonas sp. Chem Eng Technol 38(9):1511–1518. Scholar
  71. 71.
    Logan BE (2009) Exoelectrogenic bacteria that power microbial fuel cells. Nat Rev Microbiol 7:375. Scholar
  72. 72.
    Strycharz SM, Gannon SM, Boles AR, Franks AE, Nevin KP, Lovley DR (2010) Reductive dechlorination of 2-chlorophenol by Anaeromyxobacter dehalogenans with an electrode serving as the electron donor. Environ Microbiol Rep 2(2):289–294. Scholar
  73. 73.
    Wang YZ, Wang AJ, Zhou AJ, Liu WZ, Huang LP, Xu MY, Tao HC (2014) Electrode as sole electrons donor for enhancing decolorization of azo dye by an isolated Pseudomonas sp. WYZ-2. Bioresour Technol 152:530–533. Scholar
  74. 74.
    Cui M-H, Cui D, Gao L, Cheng H-Y, Wang A-J (2016) Analysis of electrode microbial communities in an up-flow bioelectrochemical system treating azo dye wastewater. Electrochim Acta 220:252–257. Scholar
  75. 75.
    Sun Q, Li ZL, Wang YZ, Yang CX, Chung JS, Wang AJ (2016) Cathodic bacterial community structure applying the different co-substrates for reductive decolorization of Alizarin Yellow R. Bioresour Technol 208:64–72. Scholar
  76. 76.
    Kong F, Wang A, Cheng H, Liang B (2014) Accelerated decolorization of azo dye Congo red in a combined bioanode-biocathode bioelectrochemical system with modified electrodes deployment. Bioresour Technol 151:332–339. Scholar
  77. 77.
    Mahmood S, Khalid A, Arshad M, Mahmood T, Crowley DE (2016) Detoxification of azo dyes by bacterial oxidoreductase enzymes. Crit Rev Biotechnol 36(4):639–651. Scholar
  78. 78.
    Liu G, Zhou J, Wang J, Zhang X, Dong B, Wang N (2015) Reductive decolorization of azo dye by bacteria. In: Singh SN (ed) Microbial degradation of synthetic dyes in wastewaters. Springer, Cham, pp 111–133. Scholar
  79. 79.
    Fang Z, Cao X, Li X, Wang H, Li X (2017) Electrode and azo dye decolorization performance in microbial-fuel-cell-coupled constructed wetlands with different electrode size during long-term wastewater treatment. Bioresour Technol 238:450–460. Scholar
  80. 80.
    Gao S-H, Peng L, Liu Y, Zhou X, Ni B-J, Bond PL, Liang B, Wang A-J (2016) Bioelectrochemical reduction of an azo dye by a Shewanella oneidensis MR-1 formed biocathode. Int Biodeter Biodegr 115:250–256. Scholar
  81. 81.
    He Z, Zhang P, Wu L, Rocha AM, Tu Q, Shi Z, Wu B, Qin Y, Wang J, Yan Q, Curtis D, Ning D, Van Nostrand JD, Wu L, Yang Y, Elias DA, Watson DB, Adams MWW, Fields MW, Alm EJ, Hazen TC, Adams PD, Arkin AP, Zhou J (2018) Microbial functional gene diversity predicts groundwater contamination and ecosystem functioning. mBio 9 (1). doi:
  82. 82.
    Yun H, Liang B, Qiu J, Zhang L, Zhao Y, Jiang J, Wang A (2017) Functional characterization of a novel amidase involved in biotransformation of triclocarban and its dehalogenated congeners in Ochrobactrum sp. TCC-2. Environ Sci Technol 51(1):291–300. Scholar
  83. 83.
    Ishii S, Suzuki S, Norden-Krichmar TM, Tenney A, Chain PS, Scholz MB, Nealson KH, Bretschger O (2013) A novel metatranscriptomic approach to identify gene expression dynamics during extracellular electron transfer. Nat Commun 4:1601. Scholar
  84. 84.
    Eddie BJ, Wang Z, WJt H, Leary DH, Malanoski AP, Tender LM, Lin B, Strycharz-Glaven SM (2017) Metatranscriptomics supports the mechanism for biocathode electroautotrophy by “Candidatus Tenderia electrophaga”. mSystems 2(2):e00002–e00017. Scholar
  85. 85.
    Coyotzi S, Pratscher J, Murrell JC, Neufeld JD (2016) Targeted metagenomics of active microbial populations with stable-isotope probing. Curr Opin Biotechnol 41:1–8. Scholar
  86. 86.
    Kowalczyk A, Eyice O, Schafer H, Price OR, Finnegan CJ, van Egmond RA, Shaw LJ, Barrett G, Bending GD (2015) Characterization of para-nitrophenol-degrading bacterial communities in river water by using functional markers and stable isotope probing. Appl Environ Microbiol 81(19):6890–6900. Scholar
  87. 87.
    Ziels RM, Sousa DZ, Stensel HD, Beck DAC (2018) DNA-SIP based genome-centric metagenomics identifies key long-chain fatty acid-degrading populations in anaerobic digesters with different feeding frequencies. ISME J 12(1):112–123. Scholar
  88. 88.
    Musat N, Musat F, Weber PK, Pett-Ridge J (2016) Tracking microbial interactions with NanoSIMS. Curr Opin Biotechnol 41:114–121. Scholar
  89. 89.
    Jiang CY, Dong L, Zhao JK, Hu X, Shen C, Qiao Y, Zhang X, Wang Y, Ismagilov RF, Liu SJ, Du W (2016) High-throughput single-cell cultivation on microfluidic streak plates. Appl Environ Microbiol 82(7):2210–2218. Scholar
  90. 90.
    Zhang D, Berry JP, Zhu D, Wang Y, Chen Y, Jiang B, Huang S, Langford H, Li G, Davison PA, Xu J, Aries E, Huang WE (2014) Magnetic nanoparticle-mediated isolation of functional bacteria in a complex microbial community. ISME J 9(3):603–614. Scholar
  91. 91.
    McAnulty MJ, Poosarla VG, Kim KY, Jasso-Chavez R, Logan BE, Wood TK (2017) Electricity from methane by reversing methanogenesis. Nat Commun 8:15419. Scholar
  92. 92.
    Liu Y, Ding M, Ling W, Yang Y, Zhou X, Li B-Z, Chen T, Nie Y, Wang M, Zeng B, Li X, Liu H, Sun B, Xu H, Zhang J, Jiao Y, Hou Y, Yang H, Xiao S, Lin Q, He X, Liao W, Jin Z, Xie Y, Zhang B, Li T, Lu X, Li J, Zhang F, Wu X-L, Song H, Yuan Y-J (2017) A three-species microbial consortium for power generation. Energy Environ Sci 10(7):1600–1609. Scholar
  93. 93.
    Lovley DR (2012) Electromicrobiology. Annu Rev Microbiol 66:391–409. Scholar
  94. 94.
    Nealson KH, Rowe AR (2016) Electromicrobiology: realities, grand challenges, goals and predictions. Microb Biotechnol 9(5):595–600. Scholar
  95. 95.
    Yu L, Yuan Y, Rensing C, Zhou S (2018) Combined spectroelectrochemical and proteomic characterizations of bidirectional Alcaligenes faecalis-electrode electron transfer. Biosens Bioelectron 106:21–28. Scholar
  96. 96.
    Strycharz SM, Glaven RH, Coppi MV, Gannon SM, Perpetua LA, Liu A, Nevin KP, Lovley DR (2011) Gene expression and deletion analysis of mechanisms for electron transfer from electrodes to Geobacter sulfurreducens. Bioelectrochemistry 80(2):142–150. Scholar
  97. 97.
    Sekar N, Wang J, Zhou Y, Fang Y, Yan Y, Ramasamy RP (2018) Role of respiratory terminal oxidases in the extracellular electron transfer ability of cyanobacteria. Biotechnol Bioeng.
  98. 98.
    Kawaichi S, Yamada T, Umezawa A, McGlynn SE, Suzuki T, Dohmae N, Yoshida T, Sako Y, Matsushita N, Hashimoto K, Nakamura R (2018) Anodic and cathodic extracellular electron transfer by the filamentous bacterium Ardenticatena maritima 110S. Front Microbiol 9:68. Scholar
  99. 99.
    Bose A, Gardel EJ, Vidoudez C, Parra EA, Girguis PR (2014) Electron uptake by iron-oxidizing phototrophic bacteria. Nat Commun 5:3391. Scholar
  100. 100.
    Zhou J, Liu W, Deng Y, Jiang YH, Xue K, He Z, Van Nostrand JD, Wu L, Yang Y, Wang A (2013) Stochastic assembly leads to alternative communities with distinct functions in a bioreactor microbial community. mBio 4(2). doi:
  101. 101.
    Strycharz SM, Woodard TL, Johnson JP, Nevin KP, Sanford RA, Loffler FE, Lovley DR (2008) Graphite electrode as a sole electron donor for reductive dechlorination of tetrachlorethene by Geobacter lovleyi. Appl Environ Microbiol 74(19):5943–5947. Scholar

Copyright information

© Springer Nature Singapore Pte Ltd. 2019

Authors and Affiliations

  • Bin Liang
    • 1
  • Mengyuan Qi
    • 2
  • Hui Yun
    • 1
  • Youkang Zhao
    • 2
  • Yang Bai
    • 2
  • Deyong Kong
    • 1
    • 3
  • Ai-Jie Wang
    • 1
    • 2
  1. 1.Key Laboratory of Environmental Biotechnology, Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijingChina
  2. 2.State Key Laboratory of Urban Water Resource and EnvironmentHarbin Institute of TechnologyHarbinChina
  3. 3.Shenyang Academy of Environmental SciencesShenyangChina

Personalised recommendations